On all regional trains – even those not marked with the special symbol – passengers are permitted to travel with a fully closed folding bicycle (even if not in the special bag) free of charge, provided that the size does not exceed 110 x 80 x 45 cm and does not cause danger or inconvenience to other passengers.

On regional trains marked with the special symbol, and limited to available spaces, each passenger can take an assembled bicycle on board with them, by purchasing the bike surcharge, which is valid until 11:59 p.m. on the date indicated on the ticket or, alternatively, another second class single ticket.

Train staff may not permit passengers to bring their bikes on board the train in the event that the transportation of your bike is deemed to be detrimental to the train service.

For detailed info please refer to the link of Trenitalia www.trenitalia.com/en/services/travelling_with_yourbike.html